The Center
has six core laboratories that help integrate the research and
provide services required by all the investigators. These cores
reduce redundancy between labs and provide services not all the
individual labs routinely perform
Genomics
Core: Co-Directed by Michael Griswold and Derek Pouchnik,
the Genomics Core provides state-of-the art genomics micro-array
technology equipment. The core laboratory provides access to the
both an Affymetrix Micro-Array apparatus and a custom array apparatus
for glass slide array of investigator initiated arrays. The Core
interacts with the Bioinformatics Core support to assist the investigators
in data analysis. Technical support is provided to perform the
analysis on the Affymetrix chips and in the production of the
custom arrays.
Proteomics
Core: Directed by James E. Bruce, the Proteomics Core provides
state-of-the art proteomics research on campus and enable much
greater insight in many biological research programs at WSU. The
objectives of this core are to provide access to technology and
expertise capable of measuring relative quantitation of proteins
from complex mixtures such as cell lysates, as well as facilitate
the identification for those proteins found to be up- or down-regulated
in response to particular stimulus, disease or treatment of interest.
In addition, this core lab will serve as a resource for consultation,
education and training in current proteomics technology, help
further accelerate biological research programs at WSU, and better
educate WSU graduates for success in the increasingly competitive
biotechnology sector.
Molecular
Biology Core: Co-Directed by Michael Griswold and Derek Pouchnik,
this Core provides DNA sequencing, protein sequencing, oligonucleotide
synthesis, peptide synthesis, and some information services to
members of the Center. A state of the art core laboratory with
all the required equipment is available.
Bioinformatics
Core: Directed by Michael Skinner, the Bioinformatics Core
www.bioinformatics.wsu.edu, is to provide computational expertise
for genomic and proteomic research studies. Staff can provide
investigators with recommendations for hardware and software necessary
to perform data analysis, storage, and management. The Bioinformatics
core provides investigators with centralized DNA/Protein, microarray,
proteomics data analysis services. The core consists of secure
high performance computers/servers and storage devices linked
to the genomics, proteomics and image analysis core. The data
generated by these core facilities is seamlessly integrated for
analysis by most commonly used analysis packages. The individual
users can also link to central servers to access their data for
analysis. WSU Bioinformatics core operates within an academic
'hybrid zone' involving Life, Computer Sciences and Engineering.
Bioinformatics core services will be accessed through the The
WSU-bioinformatics web site with links to the servers, mirror
sites for WWW sites for DNA/protein analysis and centralized servers
including a Storage Area Network (SAN) (web-based client/server
interfaces).
Plant
Transformation Core: Directed by Dr. Mechthild Tegeder, The
Plant Transformation Core laboratory provides state-of-the-art
facilities and technical support for plant researchers at WSU
to conduct plant tissue culture and transformation experiments.
As part of the Center for Integrated Biotechnology, the Core facility
performs gene transfer experiments, helps maintaining plant cultures
and assists in the development of new plant culture and transformation
protocols for investigators. All equipment and facilities needed
for plant tissue culture and transformation are provided.
Laser
Microdissection Core: Directed by Dr. Norman Lewis, the Laser
Microdissection Core laboratory provides several techniques such
as immunolocalization, in situ hybridization and reporter gene
visualization used for such purposes with differing levels of
success. Laser capture microdissection (LCM) is a relatively new
technique which makes it possible now to excise and harvest individual
cells from tissue sections. Such harvested cells can provide DNAs,
RNAs, proteins and metabolites for a plethora of applications
including gene expression profiling, as well as proteomic and
metabolite analyses of individual cell and tissue types.
Metabolomics
Core: Directed by Dr. Mark Lange, the Metabolomics Core laboratory
provides rapid, high-quality, cost-effective services for metabolomics
research at Washington State University, and for academic and
industrial collaborators. Besides routine metabolomic analysis
services, the laboratory also provides consulting for issues related
to experimental design, sample processing, and data analysis (incl.
structure elucidation and statistics). In addition, the laboratory
offers custom services for targeted metabolite profiling and metabolic
fingerprinting. The laboratory is directed by Dr. Mark Lange and
is located in Clark Hall.
