Core Laboratories
Proteomics

Director: James E. Bruce

Manger: Bill Siems

Phone: 335-2713

Email: lbb2@wsu.edu

Summary:
A core proteomics laboratory has been established to facilitate state-of-the art proteomics research on campus and enable much greater insight in many biological research programs at WSU. The objectives of this core are to provide access to technology and expertise capable of measuring relative quantitation of proteins from complex mixtures such as cell lysates, as well as facilitate the identification for those proteins found to be up- or down-regulated in response to particular stimulus, disease or treatment of interest. These capabilities will significantly extend the understanding of many research programs by providing systems-level comprehension for cases where little or nothing is known about the underlying biology of a physical process, as well as provide a much broader view of functional significance for specific genes currently known to be involved in pathways that may represent only a small subset of actual functionality. In addition, this core lab will serve as a resource for consultation, education and training in current proteomics technology, help further accelerate biological research programs at WSU, and better educate WSU graduates for success in the increasingly competitive biotechnology sector. .

Resources:
The proteomics core laboratory provides both matrix-assisted laser desorption/ionization and electrospray ionization for peptide mass mapping and sequence-based protein identification using both time-of-flight and quadrupole-based mass analyzers. Protein and peptide separation is achieved by gel electrophoresis capabilities, as well as multidimensional liquid chromatographic methods. Technical expertise is available within the core laboratory to help facilitate successful application of proteomics technology to biological research programs, as well as assist in data analysis and interpretation.