Participating Faculty
Ramond Reeves

Professor
School of Molecular Biosciences
Washington State University
Pullman, WA 99164-4660

Ph.D., 1971
University of California- Berkeley

Phone: 509-335-1948
Email: reevesr@wsu.edu

 

Research Interests:
Gene Regulation/Chromatin Structure and Function/Mammary Gland/Cancer

Research Summary:
 
The primary focus of our laboratory is the study of protein-DNA interactions involved with the regulation and repair of human genes in normal and cancerous cells. More specifically, we investigate the molecular mode of action of the HMGA group of proteins. The HMGA proteins play a vital role in cell metabolism and have been referred to as the "hubs of nuclear function" because of their involvement in such diverse processes as gene transcription, DNA replication, DNA repair and integration retroviruses (such as HIV) into the genome. We, and others, have also recently demonstrated that the HMGA proteins are causally involved in the both the cancerous transformation of normal cells and the promotion of tumor cells to more malignant states, thus making them attractive targets for new anti-cancer drugs.

Representative Publications:

Adair JE, Kwon Y, Dement GA, Smerdon MJ, Reeves R. 2005. Inhibition of nucleotide excision repair by high mobility group protein HMGA1. J Biol Chem. 280: 32184-32192.

Reeves R, Adair JE. 2005. Role of high mobility group (HMG) chromatin proteins in DNA repair. DNA Repair (Amst). 4:926-38.

Dement GA, Treff NR, Magnuson NS, Franceschi V, Reeves R. 2005. Dynamic mitochondrial localization of nuclear transcription factor HMGA1. Exp Cell Res. 307:388-401.

Miranda TB, Webb KJ, Edberg DD, Reeves R, Clarke S. (2005) Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a. Biochem Biophys Res Commun. 336(3):831-5.

Edberg DD, Adkins JN, Springer DL, Reeves R. 2005. Dynamic and differential in vivo modifications of the isoform HMGA1a and HMGA1b chromatin proteins. J Biol Chem. 280:8961-73.

Edberg DD, Bruce JE, Siems WF, Reeves R. 2004. In vivo posttranslational modifications of the high mobility group A1a proteins in breast cancer cells of differing metastatic potential. Biochem.43: 11500-15. 9.

Reeves, R. 2004. HMGA proteins: isolation, biochemical modifications, and nucleosome interactions.

Reeves, R. 2004. HMGA proteins: isolation, biochemical modifications, and nucleosome interactions. Methods Enzymol. 75:297-322.

Treff NR, D Puchnik, GA Dement, RL Britt, and R Reeves. 2004. “High Mobility Group A1a protein regulates Ras/ERK signaling in MCF-7 human breast cancer cells.” Oncogene 23: 777-85.

Henderson A, A Holloway, R Reeves, and DJ Tremethick. 2004. “Recruitment of SWI/SNF to the HIV-1 Promoter.” Mol. Cell. Biol.24: 389-397.

Dhar A, R Reeves, and NH Colburn. 2004. “Dominant-negative c-jun (TAM67) target genes: HMGA1 is required for tumor promoter-induced transformation”. Oncogene 23: 4466-76.

Beckerbauer, L., Tepe, J., Eastman, R.A., Mixter, P., Williams, R.M. and Reeves, R. 2002. Differential effects of FR900482 and FK317 on apoptosis, IL-2 gene expression, and induction of vascular leak syndrome.. Chem. & Biol. 9: 427-441.

Attema,J.L., Reeves,R.; Murray,V., Levichkin,I., Temple,M.D., Tremethick,D.J., Shannon, M.F. 2002. The human IL-2 gene promoter can assemble a positioned nucleosome that becomes remodeled upon T cell activation. J. Immunol. 169: 2466-2476.

Reeves, R. 2001. Molecular biology of HMGA proteins: Hubs of nuclear function. Gene 227: 63-81.

Reeves, R., Beckerbauer, L. 2001. HMGI/Y proteins: flexible regulators of transcription and chromatin structure.
Biochim. Biophys. Acta . 1519: 13-19.

Reeves, R., Eberg, D. and Li, Y. 2001. The architectural transcription factor HMGI(Y) promotes tumor
progression and mesenchymal transition of human epithelial cells. Molec. Cell Biol. 21: 575-594.

Pedulla, M.L., Treff, N.R., Resar, L.M.S. and Reeves, R. 2001. Sequence and analysis of the murine Hmgiy (Hmga1)
gene locus. Gene 271: 51-58.

Reeves, R., Leonard, W.J. and Nissen, M.S. 2000.Binding of HMG-I(Y) imparts architectural specificity to a positioned nucleosome on the promoter of the human interleukin-2 receptor a gene. Molec. Cell Biol. 20: 4666-4679.

Beckerbauer, L., Tepe, J.J., Cullison, J., Reeves, R. and Williams, R.M. 2000. FR900482 class of anti-tumor drugs cross-links oncoprotein HMG I/Y to DNA in vivo. Chem.. & Biol. 7: 805-812